Biochem/physiol Actions

Reversible: no

General description

Specific, irreversible inhibitor of serine proteases. Inhibits chymotrypsin, kallikrein, plasmin, thrombin, trypsin, and related thrombolytic enzymes. Stable, nontoxic alternative to PMSF (Cat. No. 52332) and DFP (Cat. No. 30967). A β-secretase inhibitor that inhibits β-amyloid peptide (Aβ) production and enhances amyloid precursor protein (sAPPU_α) secretion in several cell lines at millimolar concentrations. Use at the same molar concentrations (0.1-1.0 mM) as PMSF for most applications.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Citron, M., et al. 1996. Neuron17, 171.Ohkubo, I., et al. 1994. J. Biochem.116, 1182.Mintz, G.R. 1993. BioPharm.6, 34.Lawson, W.B., et al. 1982. Folia Haematol.109, 52.Markwardt, F., et al. 1974. Biochem. Pharmacol.23, 2247.Markwardt, F., et al. 1973. Thromb. Res.2, 343.r>Baker, B.R., and Cory, M. 1971. J. Med. Chem.14, 119.

Packaging

Packaged under inert gas

Physical form

A 50 mM (50 mg/4.17 ml) sterile-filtered solution of AEBSF, Hydrochloride (Cat. No. 101500) in H₂O.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 1 year at -20°C.

Warning

Toxicity: Standard Handling (A)